| Title |
Applying inbreeding, hybridization and mutagenesis to improve oxidative stress tolerance and longevity of the entomopathogenic nematode Heterorhabditis bacteriophora |
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Authors |
Nanette Hope Sumaya, Riddhi Gohil, Christopher Okolo, Temesgen Addis, Verena Doerfler, Ralf-Udo Ehlers, Carlos Molina |
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Publication date |
2018/01 |
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Journal |
Journal of Invertebrate Pathology |
| Volume |
151 |
| Pages |
50-58 |
| Publisher |
Elsevier |
| Abstract |
Poor shelf-life and sensitivity to environmental stress of entomopathogenic nematodes (EPNs) are traits, which deserve attention for improvement. Recently, a strong positive correlation between oxidative stress tolerance and longevity of Heterorhabditis bacteriophora dauer juveniles (DJs) has been reported. In this study, the improvement of H. bacteriophora DJ longevity was achieved by hybridization and mutagenesis. A hybrid pool deriving from two oxidative stress tolerant and long-living parental strains was generated. This hybrid AU1脙垄脗聙脗炉脙聝脗聴脙垄脗聙脗炉HU2 survived 2.6脙垄脗聙脗炉days and 18脙垄脗聙脗炉days longer than its best parent under oxidative stress and control conditions, respectively. In addition to the natural genetic variability, an EMS-mutant pool (M-OXI) with high longevity was generated and one of the derived mutagenized inbred lines (MOX-IL6) survived 5.8脙垄脗聙脗炉days and 28.4脙垄脗聙脗炉days longer than its donor line (IL3) under oxidative stress and control conditions, respectively. A genetic cross between the mutagenized inbred line and its donor line (MOX-IL脙垄脗聙脗炉脙聝脗聴脙垄脗聙脗炉IL3) still survived 2.5脙垄脗聙脗炉days and 18.5脙垄脗聙脗炉days longer than the donor line under oxidative stress and control conditions, respectively. Concerning virulence and reproductive potential, trade-off effects were not observed as a result of hybridization and mutagenesis. These results underline the potential of classical genetic approaches for trait improvement in the nematode H. bacteriophora. |
| Index terms / Keywords |
Cross-breeding, Insect pest control, Mutant nematode, Reproduction, Shelf-life, Virulence |
| DOI |
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| URL |
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Mindanao State
University - Iligan Institute of Technology